Vortex fluidics-mediated DNA rescue from formalin-fixed museum specimens

Enhanced DNA extraction and PCR amplification of mitochondrial genes from formalin-fixed museum specimens.

Fixing Formalin: A Method to Recover Genomic-Scale DNA Sequence Data from Formalin-Fixed Museum Specimens Using High-Throughput Sequencing

For 150 years or more, specimens were routinely collected and deposited in natural history collections without preserving fresh tissue samples for genetic analysis. In the case of most herpetological specimens (i.e. amphibians and reptiles), attempts to extract and sequence DNA from formalin-fixed, ethanol-preserved specimens-particularly for use in phylogenetic analyses-has been laborious and ...

Plastination of Old Formalin-fixed Specimens

This paper presents an alternative approach to the study of gross anatomy which may be pursued in Medical Schools using plastination techniques. Specimens fixed with formalin and alcohol-based techniques were utilized as the source of specimens to be plastinated; the reason for this choice was a result of the decreased organ availability in Italy due to religion and restrictive laws. The proced...

Dna Extraction from Formalin Fixed Franciscana Tissues

– The present paper reports the extraction of DNA from formalin-fixed Pontoporia blainvillei tissues. Following the Vachot and Monerot (1996) protocol, fragmented DNA (300-700bp) was extracted from more than 95% of liver and muscle samples. DNA yield in liver samples was significantly higher than in muscle samples (4.574 ± 1.169μg DNA/mg versus 0.808 ± 0.297μg DNA/mg). Similar results were obta...

Enhanced fungal DNA-extraction from formalin-fixed, paraffin-embedded tissue specimens by application of thermal energy.

Determining the etiology of invasive fungal infections (IFI) is critical for patient management as fungi vary in their susceptibility to antifungals. However, the etiology remains obscure in many cases due to negative culture results. The identification of fungal DNA by PCR in pathology blocks and sequencing it is an alternative approach to determine the cause of IFI. Previous studies identifie...